Location:TEXCHEM Lab
Preparation of Nannochloropsis sp. alginate beads
3 % sodium alginate and the nannochloropsis sp. was added into a 100 ml beaker with constant stirring until the viscous mixture is totally mixed to give a consistent colour. The mixture was then aspirated into a 10 ml syringe, which was clamped. A 250 ml beaker filled with 2 %
calcium chloride solution was placed under the syringe. The mixture was
allowed to drip drop by drop into the calcium chloride solution from a
height of about 10 cm. The beads were allowed to stand in the calcium
chloride solution for about 15 min so as to allow them to harden. The
beads were then removed by straining through a tea strainer. The beads
were washed with water and irregular shaped beads and those with trapped
air bubbles were removed. The beads were then transferred into another
clamped 10 ml syringe to about the 10 ml marking. 
End product: Nannochloropsis sp. Alginate beads
